Distribución del componente secretor en glándulas salivales de alcohólicos / Secretory component distribution in salivary glands of alcoholic subjects

En los pacientes alcohólicos se han reportado, además de una serie de alteraciones sistémicas, una pobre salud bucal, la que según algunos autores no se debería exclusivamente a una menor higiene bucal. Para probar la hipótesis de que la menor salud bucal de los pacientes alcohólicos se debe a alteraciones de la calidad de su saliva, se escogió el componente secretor como marcador de cambios en la secreción de las glándulas salivales de estos pacientes. Se estudiaron ocho muestras de glándulas salivales menores de labio de pacientes alcohólicos cirróticos, ocho muestras de alcohólicos no cirróticos y cuatro muestras controles. A estas biopsias se les aplicó una técnica inmunohistoquímica incubándolas con anticuerpo policlonal anti componente secretor. No se encontraron diferencias significativas en la ubicación del componente secretor al comparar los pacientes alcohólicos con los controles. Se corroboró la ubicación del componente secretor fundamentalmente en las semilunas de Gianuzzi y en conductos estriados, observándose igualmente en los bordes laterales de las células mucosas de los acinos

Immunoassays of secretory IgA and secretory component

1. A sandwich-type enzyme-linked immunosorbent (ELISA) is described for quantitation of secretory IgA (sIgA) in human serum, as well as an ELISA and a radioimmunoassay (RIA) for measurement of secretory component (SC) is human serum. Samples were reduced and alkylated prior to the measurement of SC. 2. Healthy individuals (N = 53) presented low levels of SC (median, 0.9 mg/l). The protein levels were significantly elevated when compared with the controls, in sera of women during the second (N = 31; median, 1.5 mg/l) and third (N = 35; median, 2.4 mg/l) and acute viral hepatitis (N = 25; median 2.4 mg/l). SC levels of women in the first trimester of pregnancy (N = 24; median, 0.5 mg/l) did not differ from the controls. 3. sIgA levels were also significantly elevated when sera of women in the third trimester of pregnancy (N = 41; median, 25.4 mg/l) and sera of patients with alcoholic cirrhosis (N = 32; median, 75.0 mg/l) or acute viral hepatitis (N = 38; median, 28.5 mg/l) were compared with controls (N = 49; median, 9.0 mg/l). women in the first (N = 25; median, 7.7 mg/l) and second (N = 29; median, 10.2 mg/l trimester of pregnancy did not present levels statistically different from the controls. 4. The results obtained for SC by RIA and ELISA were positively correlated (rs = 0.88; P < 0.001). sIgA levels determined by ELISA were also positively correlated with the results of RIA-SC(rs = 0.77;P<0.001) or ELISA-SC (rs = 0.79; P < 0.001). 5. The assays described are specific, relatively simple to perform, and can be useful for the study of the secretory system

Incidental discovery of an unusual secretor status of patient group A2B with anti-A1 antibody in the serum.

A variant of AB blood group (A2B) in a patient is described. Anti-A1 agglutinin was demonstrated in the serum and the saliva of secretor contained BH but no A substance. This is the first report of abnormality regarding secretor status of group A2B, individual from Bangladesh.